![]() Therefore, LFCs should only be used to provide a sterile environment and never for work with infectious/biohazardous materials.Īll biological safety cabinets are required to have an annual inspection and certification if used for primary containment and personal protection against biohazards. The vertical flow clean benches are useful for certain manipulations of clean materials (e.g., pouring agar plates, etc.). The horizontal flow clean benches are used in clinical, pharmaceutical, and laboratory facilities without toxic, infectious, radioactive, or sensitizing materials. Laminar flow cabinets do not provide personnel or environmental protection. Laminar flow cabinets are different from biological safety cabinets in that they provide product protection ONLY by either providing a horizontal or vertical positive pressure flow air environment. ![]() The cabinet is usually made of stainless steel with no gaps or joints where spores may collect. Air is drawn through a HEPA filter and blown in a smooth, laminar flow towards the user or towards the work surface. Class III cabinets are safe for work requiring Biosafety Level 1, 2, 3, or 4 containment.Ī laminar flow cabinet or laminar flow hood or clean bench is a carefully enclosed bench designed to prevent contamination of research materials such as semiconductor wafers, biological samples, or any particle-sensitive device. The exhaust air is treated with either double HEPA filtration or HEPA filtration and incineration. The cabinet is maintained under negative pressure and supply air is drawn in through HEPA filters. The cabinet has a transfer chamber that allows for sterilizing materials before they leave the glove box. Class IIIĪ Class III biological safety cabinet (also called a glove box) is defined as a totally enclosed, ventilated cabinet with leak-tight construction and attached rubber gloves for performing operations in the cabinet. The most common type of BSC at Auburn University is a Class II/A2 cabinet. ![]() ![]() Like Class I cabinets, Class II cabinets are safe for work using agents requiring Biosafety Level 1, 2, or 3 containment. The types include A1, A2, B1, B2, and C1. These cabinets are further differentiated by types based on construction, airflow, and exhaust systems. Class II BSCs are designed with an open front with inward airflow (personnel protection), downward HEPA-filtered laminar airflow (product protection), and HEPA-filtered exhaust air (environmental protection). Class IIĪ Class II biological safety cabinet is defined as a ventilated cabinet for personnel, product, and environmental protection for microbiological work or sterile pharmacy compounding. Class I cabinets are safe for use with agents requiring Biosafety Level 1, 2, or 3 containment. Class I cabinets have a similar airflow pattern to a fume hood, but they also have a HEPA filter at the exhaust outlet. They use non-recirculated airflow away from the operator. Class I cabinets do not offer product protection from contamination. ![]() Laboratory Safety Program the video to learn more about how biological safety cabinets work to protect you.Ī Class I biological safety cabinet is defined as a ventilated cabinet for personnel and environmental protection. In addition to ensuring safe BSC practices within laboratories, Auburn University Risk Management and Safety coordinates the certification, relocation, decontamination, and disposal of all biological safety cabinets and laminar flow cabinets on campus. All work with potentially infectious agents should be completed inside of a biological safety cabinet. This is achieved by air intake and recirculation that is filtered prior to exhausting to the cabinet exterior. A Biological Safety Cabinet (BSC) is a ventilated cabinet that uses a combination of High-Efficiency Particulate Air (HEPA) filtration, laminar airflow, and containment to provide personnel, product, and environmental protection from biohazardous agents. ![]()
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